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http://cimav.repositorioinstitucional.mx/jspui/handle/1004/1708| Validation of method for the separation and quantification of Arsenic species in human urine samples by HPLC/ICP-MS | |
| LUZ OLIVIA LEAL QUEZADA | |
| Acceso Abierto | |
| Sin Derechos Reservados | |
| Arsenic species | |
| The importance of arsenic speciation is being increasingly recognized by the scientific community because intrinsic arsenic species are known to exhibit differences in mobility, bioavailability and toxicity. A variety of organic and inorganic species have been identified in environmental and biological samples. The aim of this study was to determine the optimal operating parameters for chemical speciation of arsenic in human urine samples. HPLC/ICP-MS technique was employed to determine arsenobetaine (AsB), dimethyl arsenic acid (DMA), monomethyl arsenic acid (MMA), arsenite [As (III)] and arsenate [As (V)] in human urine samples. The separation was carried out on the Hamilton PRP X100 column, using two mobile phases: phase A containing 10 mM ammonium carbonate and phase B containing 20 mM ammonium carbonate at pH 8.83-9.35. The performance of the method was evaluated in terms of linearity, precision, accuracy and detection and quantification limits. The results demonstrate that the HPLC/ICP-MS methodology proposed is a sensitive, reproducible and accurate technique for the determination of arsenic species at trace levels in human urine. | |
| 2011 | |
| Memoria de congreso | |
| Inglés | |
| OTRAS | |
| Versión revisada | |
| submittedVersion - Versión revisada | |
| Aparece en las colecciones: | Artículos de Congresos |
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| Fichero | Tamaño | Formato | |
|---|---|---|---|
| Artículo publicado_Moreno_ICOBTE 2011.pdf | 34.35 kB | Adobe PDF | Visualizar/Abrir |