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DEVELOPMENT OF HA - nAg COMPOSITE COATING FROM GREEN PROCESS FOR HIP APPLICATIONS
VICTOR MANUEL OROZCO CARMONA
ANTONIO SERGUEI LEDEZMA PEREZ
Acceso Abierto
Sin Derechos Reservados
Biological hydroxyapatite
Silver nanoparticles
In this study, biological hydroxyapatite was obtained from bovine bones by thermal process. Then 0 and 1 % nano-silver synthesized by Opuntia ficus (nopal) containing biological hydroxyapatite coatings on a Ti6Al4V substrate were developed by atmospheric plasma spray (APS), and their antibacterial efficiency was evaluated in the following bacterial strains: Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa according to the JIS Z2801:2000 standard “Antimicrobial product- Test for antimicrobial activity and efficacy”. Ion release test shows that the coating delivered silver ions below 0.05 ppm; as well as calcium, potassium, magnesium and sodium ions (it are cell growing promoters). Scanning electron microscopy (SEM) showed that nano-silver particles (nAg) were distributed on the coating surface. Energy-dispersive X-ray spectroscopy (EDX) shows that apatite deposition occurs day by day, maintaining a Ca/P rate between 2.12 and 1.45. Biocompatibility properties were evaluated with Allium cepa, lymphocytes (fresh blood) and mouse osteoblast cells (MC3T3) by Allium cepa assay, single cell gel electrophoresis assay and Tali image cytometry.
2016
Memoria de congreso
Inglés
OTRAS
Versión revisada
submittedVersion - Versión revisada
Aparece en las colecciones: Artículos de Congresos

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